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1、每个孔上方的透明区域不含粘合剂，且易于刺穿
2、透明区域和端部标签有助于良好的校准和精确的定位
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文献和实验The Effects of Calcium-Free Seawater on the Development of Sea Urchin Embryos
urchins * Fertilization of gametes * Calcium-Free from Fertilization * Using a sterile pipette, transfer fertilized eggs to a shallow finger bowl. * Add calcium-free seawater, allow eggs to settle, than remove excess liquid and replace
, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus
Polycrystalline thin films--质谱样品准备
concentrations as the droplet dries.The recipe given below results in reproducible polycrystalline films for most matrices,although it can be difficult to use gentisic acid because of its high solubility in water.The method has been shown to gi ve useful signals
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