Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System
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Unnatural amino acid mutagenesis allows us to introduce unnatural α-amino acids into internal positions of proteins in response to expanded codons such as amber and four-base codons. To improve the unnatural amino acid mutagenesis, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus of proteins in a cell-free translation system.
![(2S)-6-amino-2-[(2R)-2-({[(2S)-2-[(2R)-2-[(2S)-2-amino-3-(1H-imidazol-4-yl)propanamido]-3-(1H-indol-3-yl)propanamido]-N'-[(4-fluorocyclohexyl)methyl]propanehydrazido]sulfonyl}amino)-3-phenylpropanamido]hexanamide，Moligand™，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/239/2939736506151833081.jpg!wh200)
