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- HZ0388
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- 详细信息
- 询价记录
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pNZ8148
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μg
pNZ8148
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0388 | pNZ8148 | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | PnisA |
|---|---|
| 复制子: | repA，repC |
| 终止子: | T |
| 质粒分类: | 乳酸杆菌表达质粒 |
| 质粒大小: | 3167bp |
| 原核抗性: | 氯霉素chloramphenicol |
| 筛选标记: | 氯霉素chloramphenicol |
| 克隆菌株: | MC1061 |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 乳酸杆菌 |
| 5'测序引物: | 根据序列设计 |
质粒简介
The pNZ8148 vector contains an origin of replication (ORI), the gene for the resistance to chloramphenicol, two genes for the replication proteins repA and repC, the nisin-inducible promoter (P nisA), and the transcription terminator (T). The gene, tagged with a Strep-tag II (STREP) followed by a stop codon (*), can be inserted between the necessary NcoI site and another endonuclease site from the multicloning site (MCS) such as PstI, SphI, KpnI, SpeI, XbaI, SacI and HindIII.
The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.
pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).
The replicons of the vectors pNZ8008, pNZ8148, pNZ8149 and pNZ8150 are identical and came originally from the Lactococcus lactis plasmid pSH71. However, this replicon has a broad host range. Plasmids with this replicon can replicate in many Gram-positive bacteria, such as Lactobacillus plantarum and Streptococcus thermophilus.
pNZ8148 – In this vector the nisA promoter is followed by an NcoI site for translational fusions at the ATG. It contains a terminator after the MCS. Sequence adaptation for cloning in NcoI can result in a change in the second amino acid of a protein (Mierau and Kleerebezem, 2005).
质粒图谱
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