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25T
产品编号:TBDIM025137
产品名称: Mouse Th1/Th2 Intracellular Cytokine Kit
产品规格:25T
产品价格:¥5680.00元
小鼠TH1/TH2细胞内因子检测试剂盒
Mouse TH1/TH2 Identification Kit is designed to enable a researcher a quick and easy way to detect IFN-γ and IL-4 expression in mouse TH1 and TH2 CD4+ T-cells.
Mouse TH1/TH2 Identification Kit contains a PE conjugated antibodies to IFN-γ and IL-4 as well as a PerCP conjugated antibody to CD4. The kit also includes optimized cytokine blocking reagents and buffers to aid in identifying TH1 and TH2 CD4+ T-cell subsets in ex vivo lymphocyte populations or to monitor TH1 and TH2 CD4+ T-cell differentiation in culture. In addition, Millipore has added a fixable viability dye that eliminates false positive data resulting from cytokine staining on dead cells that can occur in long term cultures. The TBD Mouse TH1/TH2 Identification Kit contains sufficient reagents for 25 3-color tests. Detailed assay instructions are included to assist in analysis and to ensure that the correct cell concentration is obtained during acquisition of sample data. This kit is not designed to be used in the analysis of whole spleen cells in SJL mice.
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文献和实验的作用,Th1/Th2 平衡的失调与多种疾病的发生发展和预后有着密切的关系。目前已发现许多感染性疾病、自身免疫性疾病、过敏性疾病以及移植排斥反应等都与Th1/Th2 平衡有关。因此建立稳定有效的细胞内因子检测方法对于准确说明Th1/Th2细胞的作用非常重要。目前检测的主要方法有酶联免疫法、ELISPOT法、荧光定量法及原位杂交等方法。流式细胞术检测细胞内因子是一种相对快速、重复性好并能准确定量的方法。文章参照国内外的一些文献,对于人和小鼠Th1和Th2细胞的流式检测方法进行了探索,并比较了两者之间
/Th2关系的研究进展较快。CCR3[16]、CCR4[17]选择性表达在人类或小鼠的Th2细胞,CCR5[18]、CXCR4[19]选择表达在 Th1细胞。也有报道膜表面IFN-γ受体β链可选择性地表达在 Th1细胞[20]。 二、Th1/Th2细胞的鉴定 尽管目前提出了较多的 Th1/Th2细胞表面标志,但根据淋巴细胞因子谱的异同识别Th1和Th2细胞仍然是目前最有效的手段,特别是细胞内因子标记的流式细胞技术。近来由于细胞因子ELISA试剂盒的不断涌现,为研究Th1/Th2细胞因子谱
,lncRNA FR215775可能通过促进CD4+T细胞增殖和分化参与AR,为找到AR发病机制提供了重要的见解和潜在的治疗靶点。 Figure 3 LncRNA FR215775基因敲除对Th2细胞功能和分化的影响 案例四 客户单位:同济大学附属第十人民医院 文章标题:ETS-1 facilitates Th1 cell-mediated mucosal inflammation in inflammatory bowel diseases through upregulating
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