EGFR/MAPK Activation Detection kit includes two directly conjugated phospho specific antibodies: pEGFR(Tyr1173)-Alexa Fluor 488 and pERK (Thr202/Tyr204, Thr185/Tyr187)-PE, along with optimized fixation, permeabilization, wash and assay buffers to provide researchers the ability to detect the activation of EGFR and ERK at the receptor and downstream signaling level.