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- 详细信息
- 技术资料
- 保存条件:
-20℃
- 保质期:
半年
- 英文名:
pEYFP-Actin
- 库存:
200
- 供应商:
沪震生物
- 规格:
5μl
pEYFP-Actin
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0146 | pEYFP-Actin | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | CMV |
|---|---|
| 复制子: | pUC ori,f1 ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 哺乳细胞,荧光蛋白报告载体 |
| 质粒大小: | 5820bp |
| 原核抗性: | Kan |
| 筛选标记: | Neo |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃,有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导,瞬时表达 |
| 5'测序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
pEYFP-Actin encodes a fusion of the enhanced yellow fluorescent protein (EYFP) and human cytoplasmic β-actin. EYFP is an enhanced yellow-green variant of the Aequorea victoria green fluorescent protein (GFP). The EYFP gene contains the four amino acid substitutions previously published as GFP-10C: Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. It also contains the Phe-64 to Leu mutation of GFPmut1. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 513 nm, the Em of EYFP is 36,500 cm–1M–1 and the fluorescence quantum yield is 0.63, resulting in a bright fluorescent signal. The fluorescence observed is roughly equivalent to that from EGFP
质粒图谱
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载体pEYFP-Actin
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