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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
半年
- 英文名:
Super PiggyBac Transposase (PB200PA-1)
- 库存:
200
- 供应商:
沪震生物
- 规格:
5μl
Super PiggyBac Transposase (PB200PA-1)
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0179 | Super PiggyBac Transposase (PB200PA-1) | 20μl |
¥请询价 |
使用说明
基本信息
| 启动子: | T3,T7 |
|---|---|
| 复制子: | pUC ori,f1 ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 哺乳细胞,转座子载体 |
| 质粒大小: | 8531bp |
| 原核抗性: | Amp |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃,有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导,瞬时表达 |
| 5'测序引物: | M13R:CAGGAAACAGCTATGACC |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
质粒图谱
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文献和实验The PiggyBac (PB) transposon system was originally derived from the cabbage looper moth Trichoplusia ni and represents one of the most promising transposon systems to date. Engineering of the PB transposase enzyme (PBase) and its cognate
Transgene-Free Production of Pluripotent Stem Cells Using piggyBac Transposons
independent and integrate stably into the target genome, yet benefit from the unique characteristic of seamless removal mediated by transient expression of piggyBac transposase. Thus, piggyBac transposition provides an effective means to generate human
Sleeping Beauty (SB) transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat (IR/DR) sequences that result in precise integration into a TA dinucleotide. The transposon
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