| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCAMBIA1381 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 10658 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | GUS、6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
- ¥1500
- ZYbscience
- 中国/美国
- ZY
- 2025年07月15日
11 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCambia1381
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCambia1381 10657 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-1381, complete sequence.
ACCESSION AF234302
VERSION AF234302.1 GI:7638091
KEYWORDS .
SOURCE Binary vector pCAMBIA-1381
ORGANISM Binary vector pCAMBIA-1381
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..10657
/organism="Binary vector pCAMBIA-1381"
/mol_type="other DNA"
/db_xref="taxon:118394"
CDS 2..1855
/note="gusA (N358Q) His6"
/codon_start=1
/product="GUSA (N358Q); hexaHis tagged"
/protein_id="AAF65357.1"
/db_xref="GI:7638092"
misc_feature complement(1088..1090)
/note="N358Q glycosylation mutation"
misc_feature 1884..2136
/note="nos (nopaline synthase) 3'UTR (polyA signal)"
misc_feature 2174..2199
/note="right border T-DNA repeat"
misc_feature complement(3240..4240)
/note="STA region from pVS1 plasmid"
rep_origin complement(4833..5833)
/note="pVS1-REP; replication origin from pVS1"
misc_feature complement(6243..6503)
/note="bom site from pBR322"
rep_origin complement(6643..6923)
/note="pBR322 origin of replication"
CDS complement(7214..8008)
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/codon_start=1
/product="aminoglycoside phosphotransferase"
/protein_id="AAF65358.1"
/db_xref="GI:7638093"
misc_feature 8433..8458
/note="left border repeat from C58 T-DNA"
misc_feature 8525..8733
/note="CaMV 3'UTR (polyA signal)"
CDS complement(8749..9774)
/note="hptII (hygromycin resistance) gene"
/codon_start=1
/product="hygromycin phosphotransferase"
/protein_id="AAF65359.1"
/db_xref="GI:7638094"
promoter complement(9810..10590)
/note="CaMV35S2; CaMV 35S promoter, duplicated"
ORIGIN
1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg
1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag
2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
4501 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc
4561 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
4621 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
4681 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
4741 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
4801 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
4861 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
4921 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
4981 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
5041 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
5101 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
5161 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
5221 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
5281 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
5341 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
5401 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
5461 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
5521 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
5581 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
5641 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
5701 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
5761 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
5821 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
5881 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
5941 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
6001 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
6061 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
6121 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
6181 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
6241 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
6301 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
7081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
7141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
7201 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
7261 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
7321 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
7381 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
7441 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
7501 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
7561 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
7621 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
7681 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
7741 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
7801 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
7861 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
7921 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
7981 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
8041 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
8101 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
8161 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
8221 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
8281 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
8341 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
8401 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
8461 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
8521 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
8581 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
8641 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
8701 atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct atttctttgc
8761 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
8821 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
8881 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
8941 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
1.常用载体分类：2,常用载体元件介绍：(1) 启动子：启动子是位于结构基因5'端上游的DNA序列，能活化RNA聚合酶，使之与模板DNA准确的结合并具有转录起始的特异性。启动子主要分为广谱启动子和特异性启动子。广谱启动子为在各种组织、细胞上广泛表达的启动子；特异性启动子为在特异性组织或者细胞类型上表达的启动子。常用广谱启动子有CAG，CMV，EF1a，PGK等，还包含启动RNA的III型启动子U6和H1启动子；常用特异性启动子如成熟神经元特异性启动子hSyn，投射神经元特异性启动
恰当的选择固相载体和连接分子是固相反应的重要组成部分. 固相载体和连接分子都应该在多种遗传反应过程期间是保持稳定, 而且产品的切除应该是在温和的条件下实现. 本章将介绍各种不同的载体和连接分子. 如果反应后可以用过滤的方法去除, 从理论上讲, 任何材料都可用作固相载体. 聚乙烯, 聚苯乙烯-外涂聚乙烯薄层, 平滑的纸或棉花等都可以. 最常用的载体是: 聚苯乙烯(PS), 聚乙二醇(PEG), Tentagel (TG), 和PEGA (聚乙二醇-聚丙
zhangyuxianggx 各位大侠好！现有pcDNA3-mCherry和pGEM-hgene两个东西在滤纸上，老师就说让构建载体，具体是什么意思啊？我理解的是一个真核表达载体和构建好的pGEM质粒，但是不知下一步该怎么做？恳请各位好心的大侠指点一二。 shylook 直接插入cDNA吧 zhangyuxianggx 那个pGEM就是已经插入了DNA的载体。请问这两个东西溶下来以后
技术资料暂无技术资料 索取技术资料
载体
¥1500
