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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
How to make DEPC-treated water and Tris Buffer
to remove any trace of DEPC. Notes: How to make DEPC-treated Tris buffers: DEPC will react with primary amines and cannot be used directly to treat Tris buffers. DEPC is highly unstable in the presence of Tris buffers
Role of TRIS in DNA extraction-Molecular Biology
i want to know why exactly tris is used as a buffer when we can use any inorganic salt also to reach pH 8 so what is the function of tris? -------------------------------------------------------------------------------- The pH
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