pRevTRE产品信息

pRevTRE产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0468	pRevTRE		四环素调控系统	1000	现货

产品参数

Promotor: LTR
Size: 6500
Selectable Marker: Hygromycin
Constitutive: Inducible
Viral/Non-Viral: Retroviral

载体描述

pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tet-response element (TRE). This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV) as described. The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5' viral LTR controls expression of the transcript that contains Y⁺ (the extended viral packaging signal) and the hygromycin resistance (Hyg^r) gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously. pRevTRE also includes the E. coli Amp^r gene for antibiotic selection in bacteria.　

The complete RevTet-Off and RevTet-On Systems also include the control vector pRevTRE-Luc, which was constructed by cloning the firefly luciferase gene into the HindIII/Cla I sites in the MCS of pRevTRE.

载体序列

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