pGL3-promoter产品信息

pGL3-promoter产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0124	pGL3-promoter		启动子增强子报告载体	1000 800	现货

产品参数

载体用途：启动子增强子报告载体

原核抗性：Ampicillin（氨苄青霉素）

真核筛选标记：无

启动子：SV40 promter

表达水平：中等

测序引物：RVprimer3（5'-CTAGCAAAATAGGCTGTCCC-3'）

载体拷贝数：高

载体描述

The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, and contains a modified coding region for firefly (Photinus pyralis)luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.

The pGL3-Promoter Vector contains an SV40 promoter upstream of the luciferase gene. DNA fragments containing putative enhancer elements can be inserted either upstream or downstream of the promoter-luc+ transcriptional unit.

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