pIRES-hyg3产品信息

pIRES-hyg3产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0049	pIRES-hyg3		哺乳动物表达载体	1000	现货

产品参数

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：CMV
Cloning Method：Restriction Enzyme/MCS
Constitutive or Inducible System：Constitutive

载体描述

IRES Bicistronic Expression Vectors allow you to rapidly and efficiently select positive clones that express your target protein. They include a single cassette that expresses both your gene of interest and a selection marker or reporter gene from a single promoter, so that virtually all transfected cells expressing the selection marker also express your gene of interest. With these vectors, you can screen fewer colonies to locate clones expressing high levels of your protein. The pIRES Vectors contain the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA. Ribosomes can enter the bicistronic mRNA either at the 5' end to translate the gene of interest, or at the ECMV IRES to translate the selection marker or a reporter gene. All IRES Selection Vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene, and allows preferential selection of cells expressing high levels of your protein of interest.

载体序列

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