pCEP4产品信息

pCEP4产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0021	pCEP4		哺乳动物表达载体	1000 900	现货

产品参数

载体用途：哺乳动物表达载体

质粒大小：10186bp

原核抗性：Ampicillin（氨苄青霉素）

真核筛选标记：Hygromycin（潮霉素）

启动子：CMV promter

标签：无

表达水平：高

5' 测序引物：CMV-f（5'-CGCAAATGGGCGGTAGGCGTG-3'，通用测序引物，测序公司免费提供）

3' 测序引物：EBV-r（5'-GTGGTTTGTCCAAACTCATC-3'）

载体拷贝数：高

载体描述

pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.

pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.

质粒图谱