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- bsm-33019M
- 2025年10月16日
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产品信息以Bioss网站为准
- 规格：
100ul/500ul/200ul/1000ul
| 规格： | 100ul | 产品价格： | ￥580.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥2280.0 |
| 规格： | 200ul | 产品价格： | ￥980.0 |
| 规格： | 1000ul | 产品价格： | ￥4680.0 |
| 产品编号 | bsm-33019M |
| 英文名称 | GFP Mouse mAb |
| 中文名称 | 绿色荧光蛋白单克隆抗体 |
| 英文别名 | Green fluorescence protein; |
| 中文别名 | 绿色荧光蛋白 GFP; 绿色荧光蛋白 (GFP); |
| 产品应用 | WB=1:50000-500000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant Green Fluorescence Protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > GFP |
| 亚基 | Monomer. |
| 组织特异性 | Photocytes. |
| 翻译后修饰 | Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. |
| 相似性 | Belongs to the GFP family. |
| 功能 | Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enhanced Blue Fluorescent Protein (EBFP) emits a strong blue fluorescence. EBFP can be used as protein "tags" to study the sub-cellular localization of proteins and/or their translocation upon stimulation or as markers for transfection in transient and stable expression systems. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:50000-500000} |
| {ELISA} | {1:1000-5000} |
GFP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary:
Anti-GFP-Tag (bsm-33019M) at 1/1000000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 30 kD
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 2 ug
Primary: Anti-GST tag (bsm-33019M) at 1/5000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
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文献和实验[IF={{ 6.2 }}] {Wenzhao Wang. et al. The auxin-responsive CsSPL9-CsGH3.4 module finely regulates auxin levels to suppress the development of adventitious roots in tea (Camellia sinensis). PLANT J. 2024 Jul;:} {WB} {Arabidopsis thaliana,Shaancha 1 tea,Zhongcha108 tea}
[IF={{ 3.7 }}] {Chenglong Rao. et al. Burkholderia pseudomallei BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence. MSPHERE. 2024 Oct 21} {WB} {Human}
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
recombination). Transgenes have been generated using different regions of the Oct4 gene promoter to drive expression of either lacZ or GFP, which allows the activity of the Oct4 promoter to be followed in living cells and tissues. The bacterial LacZ gene encodes
Autophagosomes in GFP-LC3 Transgenic Mice
. The most widely used marker for autophagosome is LC3, a mammalian homolog of Atg8. To analyze autophagy in whole animals, we generated GFP-LC3 transgenic mice and describe here how we determine the occurrence of autophagy in vivo using this mouse model.
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