Sample:
Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-Strep-Tag II (bs-33016R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
- ￥1180 - 2800
- Bioss
- bs-33016R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-33016R |
| 英文名称 | Strep-Tag II Rabbit pAb |
| 中文名称 | Strep-Tag II（标签）抗体 |
| 英文别名 | Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep Tag-II; STREP II TAG. |
| 产品应用 | WB=1:500-2000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Strep-Tag |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:1000-5000} |
Sample:
Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P)
Lane 2: Negative control
Primary: Anti-Strep-Tag II (bs-33016R) at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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文献和实验相关实验
I, Eco R II, Eco RV, Hae II, Hha I, Hinc II, Kpn I, Mbo I, Msp I, Nar I, Nde II, Rsa I, Sau 3a, Sca I, Sfi I, Spe I, Sph I, Ssp I, Sst I, Stu I, and Sty I. The following enzymes are ONLY PARTIALLY heat inactivated by incubation at 65 deg.C for 10
2 100mM MgCl2 10mM DTT 10mM DTT Heat inactivation of restriction enzymes The following enzymes CAN be heat inactivated by incubation at 65 deg. C for 10 min.: Alu I, Apa I, Ava II, Bal I, Bgl I, Cvn I, Dpn I, Dra I, Eco R II, Eco RV
Studies of the Ubiquitin Proteasome System
with Nonradioactive Tag (Biotin) Alternate Protocol 10: Generation of Tagged Ubiquitin Expression Plasmids Basic Protocol 18: Immunoblotting with Anti‐Ubiquitin Generation and Purification of Rabbit Anti‐Ubiquitin Antibodies Basic Protocol 19: Generation
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Strep-Tag II Rabbit pAb(bs-33016R)-50ul/100ul/200ul
￥1180 - 2800
