VEGFA | VEGF Rabbit pAb(bs-20393R)-50ul/100ul/200ul产品图
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VEGFA | VEGF Rabbit pAb(bs-203

93R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-20393R
  • 2026年10月14日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-20393R
    英文名称VEGFA Rabbit pAb
    中文名称血管内皮生长因子A抗体
    英文别名Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor(VPF);
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Dog)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human VEGFA
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量24 kDa
    浓度1mg/ml
    储存液Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
    研究领域

    Cancer > Cancer Metabolism > Response to hypoxia

    Cancer > Growth factors > VEGF

    Cancer > Invasion/microenvironment > Angiogenesis > Angiogenic growth factors

    Cardiovascular > Angiogenesis > Growth Factors > VEGF > VEGF

    Developmental Biology > Organogenesis > Angiogenesis and vasculogenesis

    Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Co-factors

    Metabolism > Pathways and Processes > Metabolism processes > Hypoxia

    Metabolism > Types of disease > Cancer

    Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus

    Signal Transduction > Growth Factors/Hormones > VEGF

    亚基Homodimer; disulfide-linked. Also found as heterodimer with PGF (By similarity).
    亚细胞定位Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
    组织特异性soform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
    相似性Belongs to the PDGF/VEGF growth factor family.
    功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature).

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Sample:
    _x000D_ Cerebellum(Rat) Lysate at 40 ug
    _x000D_ Kidney(Rat) Lysate at 40 ug
    _x000D_ Cerebrum(Rat) Lysate at 40 ug
    _x000D_ Liver(Rat) Lysate at 40 ug
    _x000D_ Primary: Anti-VEGFA (bs-20393R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 43/23 kD
    _x000D_ Observed band size: 23 kD
    _x000D_
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-20393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-20393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片4
    Sample:
    _x000D_ Lane 1: Human K562 cell lysates
    _x000D_ Lane 2: Human MDA-MB-231 cell lysates
    _x000D_ Primary: Anti-VEGFA (bs-20393R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 24 kDa
    _x000D_ Observed band size: 24 kDa

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    VEGFA | VEGF Rabbit pAb(bs-20393R)-50ul/100ul/200ul
    ￥1180 - 2800