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文献和实验microscope. This will allow observation of the cells without opening or disturbing the growth. 16. Make cell density determinations at 10 X magnification using a square ocular grid, as explained in Chapter One for the determination of area. 17. Plot
Cell Suspension Culture of Arabidopsis
一、实验试剂 10% (v/v) Household Bleach Callus Induction Medium Gamborg's B5 Basal Medium 0.5 g/liter MES pH 5.7 0.05 mg/liter Kinetin 0.5 mg/liter 2,4-dichlorophenoxyacetic Acid (2,4-D) 0.8% (w/v) Agar 2% (w/v) Glucose
up and blebbing. Electron micrographs show many long retraction fibers with a diameter of 0.25 - 0.5 µm running from the surface of the rounded cell body to enlarged, terminal bulb attachment points previously located on the flattened cell's undersurface.
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