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文献和实验-cell suspension. If you removed adherent cells by trypsinization, etc., resuspend them well (observe under microscope). Cell clumps should also be removed by passing cells through a cell-strainer. Note that there are reagents such as Accutase™
pad. Pull off remaining wash from each well with pipetter. Repeat an additional time if required. Allow to dry for 5-10 min at room temp. 13) With multipipetter, add 50 ul/well of 0.5% Triton X-100 (diluted in ddH2O). Allow to solubilize overnight
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
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