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文献和实验setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
Construction and Characterization of Adenovirus Vectors
) (Beckman) Rotor (swinging bucket, SW 41 Ti) (Beckman) Shaker (platform) Spectrophotometer Stirrer (magnetic) Syringe (3-cm3 ) Tubes (conical, 50-mL) Tubes (microcentrifuge, 1.5-mL) Tubes (polypropylene, capped, 13-mL
ES / MEF cell culture and electroporation of targeting construct
minutes at 37o C. After 10 minutes, the cells should be detached from the bottom. Now add 10 ml MEF media to this flask, pipette up and down to disaggregate the cells into a single cell suspension, and add the entire contents of the flask into one 150 cm
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