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上海研谨生物科技有限公司
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文献和实验Construction and Characterization of Adenovirus Vectors
the cells from the dish into the medium. Transfer to a 4-mL cryovial. x. Add 40% sucrose in PBAd to a final concentration of 4%. Vortex briefly. Use immediately or store at -80°C until use. xi
. For example, for 1 liter of E plates, make 500 mLs of 2X E salts, and 500 mLs of 2 X agar (1.5 g per 500 mLs). Use at least one flask large enough to mix the media together after autoclaving. 3. Cap with a polypropylene beaker and autoclave small batches
. For example, for 1 liter of E plates, make 500 mls of 2X E salts, and 500 mls of 2 X agar (1.5 g per 500 mls). Use at least one flask large enough to mix the media together after autoclaving. 3. Cap with a polypropylene beaker and autoclave small batches
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