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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20˚C/-80˚C
- 保质期:
24months
- 英文名:
Recombinant Crotalus adamanteus Thrombin-like enzyme crotalase
- 库存:
详询
- 规格:
详询
Host Species:Yeast
Target Name:VSPCR Uniprot ID: F8S114 Tag Info:His-tag 产品详细说明请点击:Recombinant Crotalus adamanteus Thrombin-like enzyme crotalase风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
for preparing assay reagents, performing HTS for USP14 inhibitors, and carrying out post?HTS analysis. Curr. Protoc. Chem. Biol. 4:311?330 © 2012 by John Wiley & Sons, Inc. Keywords: 26S proteasome; USP14; deubiquitinating enzyme; high
Strategies to Optimize Protein Expression in E. coli
‐287. Pinsach, J., de Mas, C., Lopez‐Santin, J., Striedner, G., and Bayer, K. 2008. Influence of process temperature on recombinant enzyme activity in Escherichia coli fed
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