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//重组蛋白Thrombin-like enzyme cro

talase;SVTLE;EC 3.4.21.74;Fibrinogen-clotting enzyme;Snake venom serine protease 2;SVSPCrotalus adamanteus Thrombin-like enzyme crotalase蛋白
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  • ¥2616
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  • CSB-YP520546DYB
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Crotalus adamanteus Thrombin-like enzyme crotalase

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Crotalus adamanteus Thrombin-like enzyme crotalase

    品名简称:

    Recombinant Crotalus adamanteus Thrombin-like enzyme crotalase protein

    货号:

    CSB-YP520546DYB

    规格:

    20μg

    价格:

    2616

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    N/A

    Uniprot No.:

    F8S114

    别名:

    Thrombin-like enzyme crotalase; SVTLE; EC 3.4.21.74; Fibrinogen-clotting enzyme; Snake venom serine protease 2; SVSP

    种属:

    Crotalus adamanteus (Eastern diamondback rattlesnake)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    Yeast

    分子量:

    28.8 kDa

    表达区域:

    25-262aa

    氨基酸序列:

    VIGGDECNINEHRFLVALYDYWSQSFLCGGTLINEEWVLTAKHCDRTHILIYVGVHDRSVQFDKEQRRFPKEKYFFDCSNNFTKWDKDIMLIRLNKPVSYSEHIAPLSLPSSPPIVGSVCRAMGWGQTTSPQETLPDVPHCANINLLDYEVCRTAHPQFRLPATSRTLCAGVLEGGIDTCNRDSGGPLICNGQFQGIVFWGPDPCAQPDKPGLYTKVFDHLDWIQSIIAGEKTVNCPP

    蛋白标签:

    N-terminal 6xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    25-35 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Thrombin-like snake venom protein that release fibrinopeptide A from fibrinogen (FGA). Shows both kinin-releasing and coagulant activities.

    功能2:

    Thrombin-like snake venom protein that release fibrinopeptide A from fibrinogen (FGA). Shows both kinin-releasing and coagulant activities.

    文献:

    Kallikrein-like activity of crotalase, a snake venom enzyme that clots fibrinogen.Markland F.S., Kettner C., Schiffman S., Shaw E., Bajwa S.S., Reddy K.N., Kirakossian H., Patkos G.B., Theodor I., Pirkle H.Proc. Natl. Acad. Sci. U.S.A. 79:1688-1692(1982)

    研究领域:

    Others

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    相关实验
    • Clot Retraction Test

      : 400 mL of PBS x1 + 10 mL of PBS-MgCl2 (MgCm2=4g/L) + 10 mL PBS-CaCl2 (CaCl2= 5.3 g/L) Working Solution of Thrombin = 7 µl of Thrombin (1250U/mL) + 1 mL of Clot Retraction Buffer ADDITIONAL INFO The Clot Retraction

    • A High‐Throughput Screening Method for Identification of Inhibitors of the Deubiquitinating Enzyme USP14

      for preparing assay reagents, performing HTS for USP14 inhibitors, and carrying out post?HTS analysis. Curr. Protoc. Chem. Biol. 4:311?330 © 2012 by John Wiley & Sons, Inc. Keywords: 26S proteasome; USP14; deubiquitinating enzyme; high

    • Thrombin Cleavage of GST-Fusion protein

      activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    //重组蛋白Thrombin-like enzyme crotalase;SVTLE;EC 3.4.21.74;Fibrinogen-clotting enzyme;Snake venom serine protease 2;SVSPCrotalus adamanteus Thrombin-like enzyme crotalase蛋白
    ¥2616