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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
Hints and precautions for the care, feeding and breeding of Neurospora
it tightly to the glass. We decided to use plain 13 X 100 mm culture tubes with cotton plugs and parafilm seals for silica gel stocks, in preference to screwcap tubes. With the latter, we could not be confident that the seal was tight, and the constricted
General Guide for Cryogenically Storing Animal Cell Cultures
to reduce these potential hazards is strongly recommended in such situations. Two types of vessels are commonly used for cryogenic storage: heat-sealable glass ampules and plastic (usually polypropylene) screw-capped vials. Both are available
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