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- 美国沃特世Waters
- 美国
- WAT097958
- 2025年07月10日
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文献和实验, and the clear side of the case. Put the case in the holder, black side of the case facing black side of the holder. Run the transfer at 100V for 1 hour.3. Meanwhile, prepare 500 ml of AC Buffer (+ Tween):50 ml glycerol (= 10% glycerol)10 ml 5M NaCl (= 100mM NaCl
paper, 2nd Scotch Brite Pad, and the clear side of the case. Put the case in the holder, black side of the case facing black side of the holder. Run the transfer at 100V for 1 hour. 3. Meanwhile, prepare 500 mL of AC Buffer (+ Tween): 50 mL
mg/ml Proteinase K in TBS (store up to 1 year at -20 C) 1 ml 100mM PMSF or 0.5 ml 250 mM Pefabloc Final concentration For 50ml Lysis Buffer 10mM Tris (pH 8.0) 500μL 1
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