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文献和实验from the kit to the column. 17. Let stand for 1 minute. 18. Centrifuge at 2880 x g for 5 minutes. 19. Add another 1 ml of water to the column. 20. Let stand for 1 minute. 21. Centrifuge at 2880 x g for 10 minutes. 22. Aliquot
Procedures for In Vitro Production of Bovine Embryos
in front of the heater) Heater Scissors (wipe with ethanol)Semen straw plunger (wipe with ethanol)Light microscope Plastic sterile Pasteur pipets Pipettor (25 ml) Pipet tips Instrument to pick-up oocytes Slide warmer (set at 38.5°C)Procedure1. Place X-plate
the molecular circuitry that regulates regenerative responses in mammals has recently attracted considerable attention because of its emerging impact on modern bioengineering, tissue replacement technologies, and organ transplantation. The liver
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