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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. 0.5% glutaraldehyde Make from a 25.0% stock immediately before use. You can buy a 25% solution (Sigma) and freeze-thaw it many times. 4.0% paraformaldyde 4 g paraformaldehyde 2 mM MgCl2 (0.2 ml of a 1 M stock) 1.25 mM EGTA (0.25 ml of a .5M
analysis of genomic DNA by southern blotting
reaction (10 ng/µl) 5 x *dATP buffer (for use with [a -32 P]dATP) containing dGTP, dCTP, dTTP, or 5 x *dCTP buffer (for use with [a -32 P]dCTP) containing dGTP, dATP, dTTP [a -32 P]dATP) or [a -32 P]dCTP) - not provided by the kit
analysis of genomic DNA by southern blotting
reaction (10 ng/µl) 5 x *dATP buffer (for use with [a -32 P]dATP) containing dGTP, dCTP, dTTP, or 5 x *dCTP buffer (for use with [a -32 P]dCTP) containing dGTP, dATP, dTTP [a -32 P]dATP) or [a -32 P]dCTP) - not provided by the kit
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