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文献和实验CSF Extract Prep for Spindle Assembly
that we find are most important for obtaining good CSF extracts that are competent for CSF spindle assembly and for cycled spindle assembly. The indicated buffer amounts are sufficient for a 4 frog prep. For sperm nucleus preparation, see above protocol. For spindle assembly
CSF Extract Prep for Spindle Assembly
CSF Extract Prep for Spindle Assembly Claire Walczak 11/95 This protocol is essentially as described by Murray (1991), Cell Cycle Extracts. In Methods in Cell Biology, B.K. Kay and B. Peng, eds. (San Diego
: The whole top part of the axiovert tilts back so that you can get at the needle, and also so that you can change slides on the stage without risking touching the needle. Remove the old needle by unscrewing the assembly that holds the needle. Be very careful here ; the pressure
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