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文献和实验Chromatin immunoprecipitation on native chromatin from cells and tissues
(ARC) are acknowledged for grant support. Back to top Procedure Nuclei Preparation from Tissues and Cells To prevent chromatin degradation by endogenous nucleases and to keep the chromatin intact, all steps of the nuclei
Imaging of Endogenous RNA Using Genetically Encoded Probes
, 1.40‐NA oil objective, a 100‐W mercury arc lamp for illumination and 50‐W xenon lamp for bleaching with a double lamp‐house system EM‐CCD camera (iXon, ANDOR Technology) to acquire cell
for staining gels (see Step 41) Universal tubing clamps (5 mm) (Spectrum Laboratories) UV lamp Vortex mixer Water bath set at 37°C METHOD Nuclei Preparation from Tissues and Cells Steps 1-8 describe the purification
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