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- 2025年07月13日
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文献和实验of one square mm, and the depth of the chamber is 0.1 mm. Thus the entire counting grid lies under a volume of 0.9 mm-cubed. Cell suspensions should be dilute enough so that the cells do not overlap each other on the grid, and should be uniformly distributed
so that a 75 x 25 x 1 mm microscope glass slide will sit flush in the chamber. 7) Create a recess for an O-ring around the underside of the top and bottom plates such that they are spaced apart by 0.02". Insert the rubber O-rings. 8)
Mini-Chamber for Regulating Gaseous Environment During Culture
1. The basic unit is constructed from a media filtration unit such as Corning ’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fastened to the end is attached
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