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文献和实验well in the past.Shearing volume should not exceed 200µlWash shearing orifices before and after each sample with the following settings:*All solutions must be filterd with a 0.2 micron filter before using.0.2 M HCl x20.2 M NaOH x3ddH2O x5Please use the Hydroshear manual pgs
a spin column at this point and measure the amount of counts incorporated, since this is the main factor determining the strength of signal on the autorad. Add about 20 µl of 50 mM EDTA to stop the reaction and run a spin column as usual. Expect about 50
about 20 µl of 50 mM EDTA to stop the reaction and run a spin column as usual. Expect about 50% of the counts to be incorporated for an average labelling. 2. Prehybridization of the blot- can do this while the probe labels. Wet the blot in a tray
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