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- 文献和实验
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- 供应商:
上海希言科学仪器有限公司
- 规格:
40个/箱
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文献和实验Endothelial Cell Systems - Instructions For Use
medium for every 5 cm2 surface area of the flask. Example: 15 ml growth medium for a 75 cm2 flask. d. If not using vented caps, loosen caps of flasks. Place the new culture vessels into a 37・ C humidified incubator with 5% CO2 and equilibrate
coating with polymer or adding a layer of metal. Place the slide into a sterile rectangular four-chamber cell culture vessel (or use tissue culture slide flask if polystyrene surface is desired). Count cells and seed 1.5 x 106 cells onto the slide in 5 ml
Basic procedures for bacteria culture
LB 2. Pick several colonies into a 12 X 75 mm Falcon tube containing a 2 ml aliquot of the respective liquid media, and incubate for 8-10 hours at 37degC with shaking at 250 rpm. 3. Transfer the 2 ml culture into an Ehrlenmeyer flask
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