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上海希言科学仪器有限公司
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500个/箱
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
, except resuspend the cells (~8-10 x 105 cells), in 2 mL of freezing medium for OP9 cells per 10-cm dish. Preferably, freeze cells within the first two to three passages. 14. Aliquot 1 mL of cell suspension per cryovial. 15
Procedures for In Vitro Production of Bovine Embryos
1. Prepare embryo culture medium (modified KSOM) at least 2 h before removing embryos/oocytes from the fertilization drops.2. Make enough 50 ml microdrops of culture medium (30 oocytes/embryos per drop) on 60 x 15 mm petri dishes and cover with mineral oil
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
Micromanipulators (2) (Narishige) Petri dishes (60 mm) (BD Falcon, 353002) Petri dishes (100 mm) (BD Falcon, 351029) Picoliter injector (Harvard Instruments, PLI-100) Platinum wires (3 x 0.35 mm and 80 x 0.3 mm) Scissors, angled iris (Fine
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