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上海希言科学仪器有限公司
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文献和实验Chick Chorioallantoic Membrane (CAM) Assay
, under aseptic conditions, onto a nonbinding surface (i.e., bacteriological Petri dish). The polymerization conditions for each substrate were identical; after mixing the growth factors and/or compounds, 40 μl were pipetted onto each mesh in a
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
Establishment of a Primary Culture
and remove the chorioallantoic membrane, exposing the embryo. Identify and carefully remove the embryo by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered
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