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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海希言科学仪器有限公司
- 规格:
500个/箱
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
tip) alternatively: Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade); 9'' Pasteur pipettes; 70% Ethanol; Alcohol burner or Bunsen burner; Sterile plastic Petri dishes (100 x 15 mm); Sterile
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
medium, then plate all onto a 10cm dia petri dish containing NeoPMEFs and 10ml medium. Label plate 2. 5. Set up following control plates. a) Take 0.167 ml (2 x 106 cells) original cell suspension _ 10cm dia petri dish containing NeoPMEF's and 10
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
. 500 uF 230V then 500 uF 240V 4. ASAP using a pasteur pipette transfer cells from curvette A to a 10 ml tube containing 4 mls medium, then plate 5 x 1 ml cells onto five 10cm dia petri dishes containing NeoPMEF's and 10 ml medium. Label plates
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