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- 美国Corning Falcon
- 美国
- 351007
- 2025年07月07日
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上海希言科学仪器有限公司
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500个/箱
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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
β-mercaptoethanol, 1000X Gibco® Mouse Embryonic Fibroblasts (Irradiated) Basic Fibroblast Growth Factor (bFGF) GlutaMAX™-I (100X) Collagenase Type IV for enzymatic passaging or StemPro® EZPassage™ Tool for mechanical
ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
, pour out 2 x petri dishes of PBS in the hood. Pregnant mouse is killed by cervical dislocation. (This is not done in the hood but on clean benchcote). Lay mouse out on its back and swab belly with 70% ethanol. With a pair of scissors (not sterile) nip
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
medium, then plate all onto a 10cm dia petri dish containing NeoPMEFs and 10ml medium. Label plate 2. 5. Set up following control plates. a) Take 0.167 ml (2 x 106 cells) original cell suspension _ 10cm dia petri dish containing NeoPMEF's and 10
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