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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海希言科学仪器有限公司
- 规格:
100个/箱
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文献和实验(containing 20 ug/ml chloramphenicol for BAC clones and 25 ug/ml kanamycin for PAC clones). This culture has a finite life at 4°C. We recommend that you streak this culture to single colonies on a LB agar plate with an antibiotic and prepare a glycerol stock
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
in Step 20) Plates, polystyrene, 96-well flat bottom, low cell binding (Corning 3474) (optional; see Step 21) Razor blade, sterile Scissors, sterilized microspring (8.5-cm) (Fine Science Tools 15009-08) Slides, eight-well chamber
. No. D8537) using a volume equivalent to half the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area
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