Falcon Cell Scrapers with 25cm
Handle and 3.0cm Blade, Sterile, Individually Packaged, 100/Case- 询价
- 美国Corning Falcon
- 美国
- 353089
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海希言科学仪器有限公司
- 规格：
100个/箱
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文献和实验(containing 20 ug/ml chloramphenicol for BAC clones and 25 ug/ml kanamycin for PAC clones). This culture has a finite life at 4°C. We recommend that you streak this culture to single colonies on a LB agar plate with an antibiotic and prepare a glycerol stock
. No. D8537) using a volume equivalent to half the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area
Subculture of Suspension Cell Lines
are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover the monolayer with trypsin. Decant the excess trypsin. Return flask to the incubator and leave
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