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上海希言科学仪器有限公司
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文献和实验RNA Isolation From Animal tissue or cell culture
实验试剂 Regents supplied by user: 1. 96-100% ethanol 2. $-Mercaptoethanol 实验设备 Equipments supplied by user: 1. RNase-free filter pipette tips 2. Microcentrifuge capable of 12,000
CO2 Incubator Mechanical Pipettor Inverted microscope Vacuum pump and flask Materials Complete cell culture medium, appropriate for the cell line Tissue culture flasks of appropriate sizes Tissue culture plates - 96
amount in your new containers . For our lab, using PtK1 cells, the standard distribution is in a 1 to 3 or 4 ratio for a new flask and 5-9 drops for a coverslip dish. Again this ratio will vary with your cell line. Use of ciprofloxacin-treated
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