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- 2025年07月11日
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上海希言科学仪器有限公司
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500个/箱
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文献和实验High efficiency LiAc transformation
4. Harvest the culture in a sterile 50 ml centrifuge tube at 1000 x g for 5 min. 5. Pour off the medium, resuspend the cells in 25 ml of sterile water and centrifuge again. 6. Pour off the water, resuspend the cells in 1.0 ml 100 mM LiAc
High Efficiency Transformation
with a micropipettor. Pipette 1.0 ml of sterile water into each tube; stir the pellet by with a micropipette tip and vortex . We like to be a gentle as possible at this step if high efficiency is important. Excessive washing washes away
High Molecular Weight Yeast Liquid DNA Preparation
temperature in a water bath. Make a crude sucrose gradient by first adding 11 ml of 20% sucrose, then 11 ml of 15% sucrose, then carefully underlaying 3 ml of 50% sucrose in a 36 ml Beckman centrifuge tube (Ultra-Clear 25x89 mm). Slowly pour
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