- 询价
- Signalway Antibody
- c06355b
- USA
- 2025年07月10日
- IHC-P
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Apolipoprotein B Antibody Biotin Conjugated
- 靶点：
ApoB
- 浓度：
1mg ml
- 应用范围：
IHC-P
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 标记物：
Biotin
- 克隆性：
Polyclonal
- 保存条件：
Store at 4°C for 12 months.
- 形态：
liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide aa 1540-1590 4563 derived from human Apolipoprotein B
- 规格：
100ul
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
for each species of primary antibody used. The biotin molecule is small and can be easily conjugated to immunoglobulin by amino substitution at alkaline pH without the loss of immunoglobulin activity. These secondary antibodies are quite inexpensive, readily
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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