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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal antibody
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB, ICC/IF, IHC
- 靶点:
Uniprot:P35354
- 抗体英文名:
COX2/Cyclooxygenase 2 Rabbit mAb
- 规格:
50ul/100ul
COX-2 antibody
COX2 antibody
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Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
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OTTHUMP00000033524 antibody
PES-2 antibody
PGG/HS antibody
PGH synthase 2 antibody
PGH2_HUMAN antibody
PGHS 2 antibody
PGHS-2 antibody
PGHS2 antibody
PHS 2 antibody
PHS II antibody
PHS2 antibody
Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
Prostaglandin endoperoxide synthase 2 antibody
Prostaglandin G/H synthase 2 antibody
Prostaglandin G/H synthase 2 precursor antibody
Prostaglandin G/H synthase and cyclooxygenase antibody
Prostaglandin G/H synthase antibody
Prostaglandin H2 synthase 2 antibody
prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
Prostaglandin-endoperoxide synthase 2 antibody
PTGS2 antibody
ptgs2a antibody
TIS10 antibody
TIS10 protein antibody
unp1239 antibody
wu:fj02a10 antibody
计算分子量:72 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:1,000-5,000
IHC: 1:100-1:5,000
ICC: 1:50-1:200
图片:

Western blot analysis of COX2 on different lysates using anti-COX2 antibody at 1/1,000 dilution. Positive control:
Lane 1: A549
Lane 2: THP-1
,

ICC staining COX2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining COX2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
,

ICC staining COX2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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文献和实验:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上,同时减少蛋白不必要降解,这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息:1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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