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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
GCDFP15 Antibody HRP Conjugated
- 靶点:
GCDFP15
- 浓度:
1mg ml
- 应用范围:
WB, IHC-P
- 宿主:
Rabbit
- 适应物种:
Mouse
- 标记物:
HRP
- 克隆性:
Polyclonal
- 保存条件:
Store at 4°C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from mouse GCDFP15
- 规格:
100ul
产品详细说明请点击:GCDFP15 Antibody HRP Conjugated
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文献和实验在 DMF 中稳定,因此,可以用 100 mL DMF 溶解 0.4 g AEC 作为贮存液备用; (2) 加 l mL AEC 溶液 至 15 mL 0.1 mol/L 的乙酸钠缓冲液(pH5.2)中,并搅拌; (3) 加 0.15 mL 3% 过氧化氢于水中。过氧化氢一般是 30% 的溶液提供,在 4℃ 可存放 1 个月; (4) 用滤纸过滤; (5) 加此溶液到标本中,并于室温孵育 10~40 min,水洗终止反应; (6) 优化:如果需要复染,只可用水溶性染料; (7) 用 Gelvatol
Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots
between negatively charged dihydrolipoic acid (DHLA)-capped CdSe-ZnS core-shell quantum dots and positively charged proteins (natural or engineered) that serve to bridge the quantum dot and antibody. This chapter details the materials and methods for synthesis
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