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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
GCDFP15 Antibody FITC Conjugated
- 靶点:
GCDFP15
- 浓度:
1mg ml
- 应用范围:
IF(IHC-P)
- 宿主:
Rabbit
- 适应物种:
Human
- 标记物:
FITC
- 克隆性:
Polyclonal
- 保存条件:
Store at 4°C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human GCDFP15
- 规格:
100ul
产品详细说明请点击:GCDFP15 Antibody FITC Conjugated
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文献和实验Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots
between negatively charged dihydrolipoic acid (DHLA)-capped CdSe-ZnS core-shell quantum dots and positively charged proteins (natural or engineered) that serve to bridge the quantum dot and antibody. This chapter details the materials and methods for synthesis
当FITC在碱性溶液中与抗体蛋白反应时,主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合,形成FITC-蛋白质结合物,即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基,一般最多能结合15~20个,一个IgG分子可结合2~8个分子的FITC,其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体,也可以根据条件采用Chadwick等标记法或Clark
of conjugated-Ig is added to the column. If 2mL of conjugated-Ig is added, discard first 1mL and keep the next 4mL. The antibody is always diluted by 2x after leaving the column. 5. When FITC coupling an antibody, you can determine the FITC to Protein ratio
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