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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Aurora A+B+C Antibody FITC Conjugated
- 靶点:
Aurora A+B+C
- 浓度:
1mg ml
- 应用范围:
IF(IHC-P)
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 标记物:
FITC
- 克隆性:
Polyclonal
- 保存条件:
Store at 4°C for 12 months.
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human Aurora A B C
- 规格:
100ul
产品详细说明请点击:Aurora A+B+C Antibody FITC Conjugated
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文献和实验. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0 b) Determine and record A280 and A492. c) Calculate
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
involves conventional 3C methodology: The chromatin is cross-linked, digested with restriction enzymes, and ligated under conditions that favor intramolecular ligation. Immediately after ligation, the chromatin is immunoprecipitated using an antibody
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