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Fermentas 逆转录试剂盒
- 英文名：
RevertAid First Strand cDNA Synthesis Kit
- 库存：
Fermentas 逆转录试剂盒
- 供应商：
研卉生物
- 规格：
200 reactions
Maxima First Strand cDNA Synthesis Kit for RT-qPCR
描述
Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. The kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT.The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C).The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.
Highlights
• High yields of full length cDNA up to 20 kb
• Efficient cDNA synthesis at wide temperature range from 42°C to 65°C
• Increased synthesis rate—complete cDNA synthesis in 15-30 minutes
• High sensitivity and specificity
Applications
• 2-step RT-PCR
• 2-step RT-qPCR
Maxima First Strand cDNA Synthesis Kit for RT-qPCR contains
• Maxima Enzyme Mix
• 5X Reaction Mix
• nuclease-free water
内容及储存
• Maxima Enzyme Mix (Maxima Reverse Transcriptase and RiboLock RNase Inhibitor)
• 5X Reaction Mix (reaction buffer, dNTPs, oligo(dT)18 and random hexamer primers)
• nuclease-free water
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文献和实验性，因而即使是最具挑战性的模板也可以进行有效的RT-PCR.。 通过SuperMix形式快速合成cDNA SuperScript™ III First—Strand Synthesis SuperMix将所有进行强大的、灵敏的第一链cDNA合成所必须的组分组合成快速、易于使用的 SuperMix形式。SuperMix形式包含 2x RT缓冲反应混合物、dNTPs、MgCl2、 以及由SuperScript™ III RT和RNaseOUT™.组成的酶混合物。也提供优化的退火缓冲
and synthesis of first-strand cDNA (ref. 60), allowing the construction of solid-phase cDNA libraries and solid-phase RT-PCR. 实验试剂 Lysis/Binding Buffer Washing Buffer A Washing Buffer B 10 mM Tris-HCl (Elution Buffer
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
and the bead-bound oligo (dT) as primer for first strand cDNA synthesis, an initial incubation at 50°C for 5 minutes is necessary before proceeding at the recommended temperature. 注意事项 1. Keep the vials of Dynabeads Oligo (dT)25
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