- ￥5600
- GeneTex
- 美国
- GTX64165
- 2025年07月24日
- WB, ICC/IF, IP
- Rabbit
- Schizosaccharomyces pombe
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant C-terminal region (1.8kb) of S. pombe Sds22
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Schizosaccharomyces pombe
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Yeast
- 目录编号：
GTX64165
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IP
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Specific to S. pombe
- 抗体英文名：
Sds22 (S. pombe) antibody
- 抗体名：
Sds22 (S. pombe) 抗体
- 规格：
100 μl
Sds22 coprecipitates with Dis2 and Sds21. Yeast extracts of wild type (wt) strain HM123 (lane 1 and 2), dis2::ura4+ deletion mutant (Δd2, lane 3), sds21::ura4+ deletion mutant (Δs21, lane 4) were immunoprecipitated followed by immunoblotting with the indicated antiserum, to detect the Sds22 or Dis2/Sds21 proteins. Lane 1 was immunoprecipitated with the appropriate preimmune serum, lane 2-4 with the anti-Sds22 serum. (a) denotes anti-Sds22 immunoblot; (b) denotes anti-D2C immunoblot. Anti-D2C crossreacts with both Sds21 and Dis2. Anti-Sds22 antiserum coprecipitates both Dis2 and Sds21 proteins in the wild type strain (lane 2b). Consistently, Sds21 alone is precipitated in the dis2 deletion mutant (lane 3b), and Dis2 alone is precipitated in the sds21 deletion mutant (lane 4b).
Sds22 subcellular localization Indirect immunofluorescence microscopy was performed by staining methanol fixed cells with (first column) anti-Sds22 antiserum, or (second column) DAPI to visualize chromosomal DNA. (a) wild type HM123; (b) HM123 carrying multicopy sds22+ plasmid pHR140-2. Anti-Sds22 antibody stains the cytoplasm as well as the non-chromosomal domain of the nucleus of a wild type strain, as shown in (a). Nuclear staining increases in strains carrying a multicopy sds22+ plasmid (b).
Immunoblot with anti-Sds22 antiserum of yeast extracts from (1) wild type strain HM123, (2) sds::ura4+ deletion mutant carrying pHR140-2. The 40kD protein band was identified by immunoblot analysis of wild-type strain using anti-Sds22 antisera (lane1). The 40 kD band is enhanced in the sds22::ura4+ disruptrion mutant strain that is rescued by the multicopy sds22+ plasmid pHR140-2 (lane2).
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文献和实验Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
follicles and at the rim of germinal centers (2 ). Consequently, Ber-H2 has been utilized as an immunotoxin by conjugating the antibody to the ribosome-inactivating toxin Saporin to treat four patients with refractory Hodgkin’s lymphoma
protein (GFP) under the control of an attenuated nmt1 promoter in Schizosaccharomyces pombe . The antibody binds to its target antigen without inhibiting protein function, allowing visualization of its intracellular location in fixed or living cells.
SDS Gel Electrophoresis of Tubulin\MAPs
(1:3 dilution of stock) 0.375 M Tris-HCl (pH 8.8) 0.1 % SDS Stacking Gel (Final Concentrations) 3 % acrylamide (1:10 dilution of stock) 0.125 M Tris-HCl (pH 6.8) 0.1% SDS Electrode
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