化学发光EMSA试剂盒Gelshift™ Chemilumi

nescent EMSA

价格¥5500

品牌activemotif
货号37341
更新日期2025年07月15日

12 年金牌会员

非放射性EMSA，简化了蛋白质-DNA绑定的筛选

The Gelshift Chemiluminescent EMSA Assay Kit provides a simple, non-radioactive assay to identify protein-DNA binding with proven reagents. In this electrophoretic mobility shift assay (EMSA), cell extracts or purified factors are incubated with biotin end-labeled probe containing the consensus binding site of interest. Samples in which the protein of interest bound the target DNA will migrate slower than DNA alone resulting in a "shift" of the labeled DNA band. For complete details, click the EMSA Method tab below.

Gelshift Chemiluminescent EMSA Assay Kit

The Gelshift Chemiluminescent EMSA Assay Kit includes all the reagents needed to study protein-DNA binding for your sample system. The kit also includes biotin-labeled and unlabeled control DNA and control nuclear extract. Click the Gel Shift tab below for data and more information; kit manuals can be downloaded under the Documents tab.

References

1. Fried, M. and Crothers, D.M. (1981) Nucleic Acids Res. 9: 6505-6525.
2. Revzin, A. (1989) BioTechniques 7: 346-354.
3. Hendrickson, W. (1985) BioTechniques 3: 198-207.

Contents & Storage

The Gelshift Chemiluminescent EMSA Kits contain 10X Binding Buffer, Biotin Control DNA, Unlabeled Control DNA, Control Nuclear Extract, Poly d(I-C), 50% Glycerol, 1% NP-40, 1 M KCl, 100 mM MgCl2, 200 mM EDTA pH 8.0 and 5X Loading Buffer, which are all stored at -20°C. Additionally, the kit includes Streptavidin HRP-conjugate, Chemiluminescent Reagent, Reaction Buffer, Blocking Buffer, 4X Wash Buffer and Substrate Equilibration Buffer which should be stored at 4°C.

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文献和实验

相关实验

非放射EMSA实验技术讨论
激活－核转运检测试剂盒仅染色p65，不染色RelB、C-Rel、p50和p52。使用本试剂盒染色后NF-κB呈红色荧光，细胞核呈蓝色荧光.思考信号转导和检测的理论我做了这样的回答(个人意见.大家可以讨论!)：这种理论上来讲可行,但是实际操作可能拿不到你想要得结果,原因很简单,凭染色来判断是很难区分程度上的差异的,就比如做WB实验,经典的还是化学发光的方法,显色的方法只能得到信号比较强的蛋白,遇到信号比较弱的蛋白就误差很大了, 第二点,并不是所有能进核的蛋白都有结合DNA
有关EMSA的问题
----------------------- xixi76120 wrote: yfyu1963 : 我目前准备做EMSA，检测猪的转录因子NFKB，E2F，我不想用同位素检测方法，听说化学发光法效果不错，我想请教一下：1 两者在实验操作中步骤有何大的区别么？2 化学发光法用特殊的远红外检测仪么？3 上述两种转录因子能用一个试剂盒作么？4 检测猪的转录因子与鼠和人的主要困难在哪里？需要注意点什么东东呢？问题多了点，谢谢
Introduction to the EMSA (Gel Shift) Technique
4 polynucleotide kinase. Alternatively, DNA can be labeled with a biotinylated or hapten-labeled dNTP, then probed and detected using an appropriately sensitive fluorescent or chemiluminescent substrate. Pierce offers a chemiluminescent EMSA system (LightShift

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