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研卉生物
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100 rxns
Gelshift™ 化学发光EMSA
非放射性EMSA，简化了蛋白质-DNA绑定的筛选
The Gelshift Chemiluminescent EMSA Assay Kit provides a simple, non-radioactive assay to identify protein-DNA binding with proven reagents. In this electrophoretic mobility shift assay (EMSA), cell extracts or purified factors are incubated with biotin end-labeled probe containing the consensus binding site of interest. Samples in which the protein of interest bound the target DNA will migrate slower than DNA alone resulting in a "shift" of the labeled DNA band. For complete details, click the EMSA Method tab below.
Gelshift Chemiluminescent EMSA Assay Kit
The Gelshift Chemiluminescent EMSA Assay Kit includes all the reagents needed to study protein-DNA binding for your sample system. The kit also includes biotin-labeled and unlabeled control DNA and control nuclear extract. Click the Gel Shift tab below for data and more information; kit manuals can be downloaded under the Documents tab.
References
1. Fried, M. and Crothers, D.M. (1981) Nucleic Acids Res. 9: 6505-6525.
2. Revzin, A. (1989) BioTechniques 7: 346-354.
3. Hendrickson, W. (1985) BioTechniques 3: 198-207.
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