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- 文献和实验
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- 保存条件:
2~8℃
- 保质期:
三年
- 英文名:
Lambda DNA/EcoR I Marker
- 库存:
1000
- 供应商:
合肥博美
- 规格:
50T/100T
| 规格: | 50T | 产品价格: | ¥168.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥304.0 |
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文献和实验Screening lambda cDNA or genomic libraries
,and this will increase the efficiency of labelling considerably. - Boil the DNA to denature it for a few minutes.Briefly spin the tube and put on ice to cool.Add 6.6 µl 5X OLB,5 µl hot dATP (50 µCi),and 2 µl Klenow. - Allow the labelling reaction to proceed at room
Recombineering/Lambda red-mediated gene replacement
Electroprate linear DNA into electrocompetent cells Grow at 37°C on chloramphenicol plates PCR verify the deletion with oligos C and D (see below for design) Detailed procedure Day 0: Start overnight culture
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques
Natural History Museum Protocol for EST sequencing from lambda zap phage plaques (by PCR of phage insert, cycle sequencing using ABI dye terminator kits and automated sequencing on an ABI 373A) (1) Core out a single
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