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- bs-0375R
- 2025年10月10日
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- 规格：
1mg/10mg
| 规格： | 1mg | 产品价格： | ￥1280.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥6400.0 |
| 产品编号 | bs-0375R |
| 英文名称 | Rabbit Anti-Guinea Pig IgM |
| 中文名称 | 兔抗豚鼠IgM |
| 英文别名 | Guinea pig Immunoglobulin M; Gpig IgM; |
| 产品应用 | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:200-1000, IF, ELISA=1:2000-10000 Not yet tested in other applications. |
| 交叉反应 | Guinea Pig |
| 抗体来源 | Rabbit |
| 免疫原 | Native Guinea pig IgM |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin M (IgM) normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:200-1000} |
| {ELISA} | {1:2000-10000} |
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文献和实验[IF={{ 4.5 }}] {Yingjun Cui. et al. Salp14 epitope-based mRNA vaccination induces early recognition of a tick bite. VACCINE. 2024 Oct;42:126304} {ELISA} {Guinea pig}
Xenoantibodies and Complement Activity Determinations in Pig-to-Primate Xenotransplantation
total endogenous complement activity; the cytolytic activity of anti-pig antibodies (APA) is also measured by hemolytic assay; an ELISA is used for quantification of anti-galactose α1,3galactose (Gal) IgG and IgM antibodies and flow cytometry
) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
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