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- bs-0375Rs
- 2025年10月10日
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| 产品编号 | bs-0375Rs |
| 英文名称 | Rabbit Anti-Guinea Pig IgM whole serum |
| 中文名称 | 兔抗豚鼠IgM抗血清 |
| 英文别名 | Guinea pig Immunoglobulin M; Gpig IgM; |
| 交叉反应 | Guinea Pig |
| 抗体来源 | Rabbit |
| 免疫原 | Native Guinea pig IgM |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | Unpurified |
| 克隆类型 | Polyclonal |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. |
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文献和实验The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)
of spleen cells is prepared. These spleen cells containing the IgM secreting plasma cells are incubated in a semisolid matrix of agar, sheep erythrocytes, and guinea pig serum as a single cell layer between a Petri dish and glass cover slip. After a 3 h
for a plant virus, tobacco mosaic virus. In 1963, Anderer (1 ) demonstrated that rabbit antibodies to an isolated hexapeptide fragment from the virus-coat protein coupled to bovine serum albumm would neutralize the infectious vn-us in culture. Two years later
Double immunofluorescence staining for BCL-6 and else.
at this section for dewaxed, antigen retrieved slides 6- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 5 minutes / wash. 7- Briefly blot the slides without letting them dry and then apply 3% human serum (health hazard!) or pig serum
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