Cela3b/Cela3b蛋白/Elastase IIIB

Research Areas

Cell Biology

Uniprot ID

Q9CQ52

Gene Names

Cela3b

Alternative Name(s)

Elastase IIIB Elastase-3B Protease E

Lead Time

3-7 business days

Organism

Mus musculus (Mouse)

Source

E.coli

Expression Region

28-269aa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Target Protein Sequence

VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN

MW

31.1 kDa

Purity

Greater than 90% as determined by SDS-PAGE.

Form

Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid form

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.